Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis performed by coating plate with recombinant HEK293 expressed, N-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10^-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
3. Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] detects Respiratory Syncytial virus type A Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% PFA.
Green: Respiratory Syncytial virus type A Nucleoprotein stained by Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-infected (–) and infected (+) HEp-2 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Respiratory syncytial virus lysate (1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1297] (GTX636711) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.